Evaluating “Helleborus cyclophyllus” extract’s cytotoxic activity against malignant and normal cell lines? preliminary evidence of gastric acid resistance, and a promising therapeutic window

Helleborus cyclophyllus is an ancient pharmaceutical plant that exhibits remarkable in vitro cytotoxic activity against malignant cell lines. In the present study, we evaluated of dried powdered rhizome’s aqueous extract both (PC3, TPC1, K1 ) and normal ( Nthy-ori 3.1) Our results demonstrated a promising therapeutic window, as lines exhibited significantly higher (x 10) IC50 than Gastric acid did not adversely affect extract’s previous incubation 1.0 M HCl result values escalation. Finally, high extracellular lactic concentration resulted amplification, whereas presence sodium lactate affected cytotoxicity, revealing possible indirect potential role for aerobic glycolysis to clinically observed inadequacy per os administration cancer treatment.